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+# Copyright 2013-2018 Lawrence Livermore National Security, LLC and other
+# Spack Project Developers. See the top-level COPYRIGHT file for details.
+#
+# SPDX-License-Identifier: (Apache-2.0 OR MIT)
+
+from spack import *
+
+
+class Trinity(MakefilePackage):
+ """Trinity, developed at the Broad Institute and the Hebrew University of
+ Jerusalem, represents a novel method for the efficient and robust de
+ novo reconstruction of transcriptomes from RNA-seq data. Trinity
+ combines three independent software modules: Inchworm, Chrysalis, and
+ Butterfly, applied sequentially to process large volumes of RNA-seq
+ reads. Trinity partitions the sequence data into many individual de
+ Bruijn graphs, each representing the transcriptional complexity at a
+ given gene or locus, and then processes each graph independently to
+ extract full-length splicing isoforms and to tease apart transcripts
+ derived from paralogous genes.
+ """
+
+ homepage = "http://trinityrnaseq.github.io/"
+ url = "https://github.com/trinityrnaseq/trinityrnaseq/archive/Trinity-v2.6.6.tar.gz"
+
+ version('2.6.6', 'b7472e98ab36655a6d9296d965471a56')
+
+ depends_on("java@8:", type=("build", "run"))
+ depends_on("bowtie2")
+ depends_on("jellyfish")
+ depends_on("salmon")
+ depends_on("perl+threads", type=("build", "run"))
+ depends_on("autoconf", type="build")
+ depends_on("automake", type="build")
+ depends_on("libtool", type="build")
+
+ # There is no documented list of these deps, but they're in the Dockerfile
+ # and we have runtime errors without them
+ # https://github.com/trinityrnaseq/trinityrnaseq/blob/master/Docker/Dockerfile
+ depends_on("blast-plus", type="run")
+ depends_on("bowtie", type="run")
+ depends_on("r", type="run")
+ depends_on("r-tidyverse", type="run")
+ depends_on("r-edger", type="run")
+ depends_on("r-deseq2", type="run")
+ depends_on("r-ape", type="run")
+ depends_on("r-gplots", type="run")
+ depends_on("r-biobase", type="run")
+ depends_on("r-qvalue", type="run")
+ depends_on("rsem", type="run")
+ depends_on("kallisto", type="run")
+ depends_on("fastqc", type="run")
+ depends_on("samtools", type="run")
+ depends_on("py-numpy", type="run")
+ depends_on("express", type="run")
+ depends_on("perl-dbfile", type="run")
+ depends_on("perl-uri-escape", type="run")
+ depends_on("r-fastcluster", type="run")
+ depends_on("r-ctc", type="run")
+ depends_on("r-goseq", type="run")
+ depends_on("r-glimma", type="run")
+ depends_on("r-rots", type="run")
+ depends_on("r-goplot", type="run")
+ depends_on("r-argparse", type="run")
+ depends_on("r-sm", type="run")
+
+ def build(self, spec, prefix):
+ make()
+ make("trinity_essentials")
+ make("plugins")
+
+ def install(self, spec, prefix):
+ install_tree('.', prefix.bin)
+ force_remove(join_path(prefix.bin, '.gitmodules'))
+ force_remove(join_path(prefix.bin, 'Butterfly', '.err'))
+ force_remove(join_path(prefix.bin, 'Butterfly', 'src', '.classpath'))
+ force_remove(join_path(prefix.bin, 'Butterfly', 'src', '.err'))
+ force_remove(join_path(prefix.bin, 'Butterfly', 'src', '.project'))
+ remove_linked_tree(join_path(prefix.bin, 'Butterfly', 'src',
+ '.settings'))
+ remove_linked_tree(join_path(prefix.bin, 'Inchworm', 'src', '.deps'))
+ remove_linked_tree(join_path(prefix.bin, 'trinity-plugins',
+ 'ParaFly-0.1.0', 'src', '.deps'))
+ force_remove(join_path(prefix.bin, 'trinity-plugins',
+ 'seqtk-trinity-0.0.2', '.gitignore'))
+ force_remove(join_path(prefix.bin, 'trinity-plugins', 'slclust', 'bin',
+ '.hidden'))
+
+ def setup_environment(self, spack_env, run_env):
+ run_env.set('TRINITY_HOME', self.prefix.bin)
+ run_env.prepend_path('PATH', self.prefix.bin.util)
+ spack_env.append_flags('CXXFLAGS', self.compiler.openmp_flag)